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Reconstitution Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reconstitution buffer  (MedChemExpress)
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The SDS–PAGE results confirm similar levels of protein <t>reconstitution</t> in each liposome preparation. The gel is one representative result from at least two independent experiments.
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0 m reconstitution buffer  (Bio-Rad)
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Bio-Rad 0 m reconstitution buffer
The SDS–PAGE results confirm similar levels of protein <t>reconstitution</t> in each liposome preparation. The gel is one representative result from at least two independent experiments.
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sterile deionized water reconstitution buffer  (China Pharmaceuticals Inc)
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The SDS–PAGE results confirm similar levels of protein <t>reconstitution</t> in each liposome preparation. The gel is one representative result from at least two independent experiments.
Sterile Deionized Water Reconstitution Buffer, supplied by China Pharmaceuticals Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reconstituted luciferase assay buffer  (Promega)
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Promega reconstituted luciferase assay buffer
Electromagnetic waves reduce infectivity of SARS-CoV-2 Virus-Like Particles (SC2-VLPs). (A) Diagram of SC2-VLP production, electromagnetic wave treatment and infectivity assay. Plasmids expressing each of the SARS-CoV-2 structural proteins (Nucleocapsid (N); Matrix (M); Envelope (E); and Spike (S)), or a plasmid encoding a packaging signal and <t>luciferase</t> transcript (Luc-PS9) were transfected into viral producer cells (HEK293T). SC2-VLPs were then collected, left unexposed or exposed to various electromagnetic wave frequencies (1–6 GHz) and used to infect target cells (HEK293T-ACE2-TMPRSS2). The next day, luciferase was measured as a readout of infectivity. Created in https://BioRender.com . (B) Western blot of pelleted Spike (S), Nucleocapsid (N), Membrane (M), Envelope (E) in unexposed virus particles. The image is representative of two independent experiments. (C) SC2-VLP infectivity assay. Frequencies (1–6 GHz) were tested over a range of exposure times (2–10 min). Data represent compiled mean +/- SD of 5 independent experiments. The luciferase signal for each frequency/exposure time was performed in triplicate. P-values indicate Wilcoxon matched-pair signed rank test of unexposed compared to exposed samples. ( n = 90 (Unexposed); n = 36 (1.0–2.5 GHz & 2.5–3.5 GHz) n = 15 (3.5–4.8 GHz), n = 33 (4.8–6 GHz), n = 18 (MW)). RLU: Renilla Luciferase Units (RLU). MW: Microwave.
Reconstituted Luciferase Assay Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10x reconstitution buffer  (Thermo Fisher)
99
Thermo Fisher 10x reconstitution buffer
Electromagnetic waves reduce infectivity of SARS-CoV-2 Virus-Like Particles (SC2-VLPs). (A) Diagram of SC2-VLP production, electromagnetic wave treatment and infectivity assay. Plasmids expressing each of the SARS-CoV-2 structural proteins (Nucleocapsid (N); Matrix (M); Envelope (E); and Spike (S)), or a plasmid encoding a packaging signal and <t>luciferase</t> transcript (Luc-PS9) were transfected into viral producer cells (HEK293T). SC2-VLPs were then collected, left unexposed or exposed to various electromagnetic wave frequencies (1–6 GHz) and used to infect target cells (HEK293T-ACE2-TMPRSS2). The next day, luciferase was measured as a readout of infectivity. Created in https://BioRender.com . (B) Western blot of pelleted Spike (S), Nucleocapsid (N), Membrane (M), Envelope (E) in unexposed virus particles. The image is representative of two independent experiments. (C) SC2-VLP infectivity assay. Frequencies (1–6 GHz) were tested over a range of exposure times (2–10 min). Data represent compiled mean +/- SD of 5 independent experiments. The luciferase signal for each frequency/exposure time was performed in triplicate. P-values indicate Wilcoxon matched-pair signed rank test of unexposed compared to exposed samples. ( n = 90 (Unexposed); n = 36 (1.0–2.5 GHz & 2.5–3.5 GHz) n = 15 (3.5–4.8 GHz), n = 33 (4.8–6 GHz), n = 18 (MW)). RLU: Renilla Luciferase Units (RLU). MW: Microwave.
10x Reconstitution Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/10x reconstitution buffer/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
10x reconstitution buffer - by Bioz Stars, 2026-03
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protein reconstitution buffer  (Thermo Fisher)
99
Thermo Fisher protein reconstitution buffer
Electromagnetic waves reduce infectivity of SARS-CoV-2 Virus-Like Particles (SC2-VLPs). (A) Diagram of SC2-VLP production, electromagnetic wave treatment and infectivity assay. Plasmids expressing each of the SARS-CoV-2 structural proteins (Nucleocapsid (N); Matrix (M); Envelope (E); and Spike (S)), or a plasmid encoding a packaging signal and <t>luciferase</t> transcript (Luc-PS9) were transfected into viral producer cells (HEK293T). SC2-VLPs were then collected, left unexposed or exposed to various electromagnetic wave frequencies (1–6 GHz) and used to infect target cells (HEK293T-ACE2-TMPRSS2). The next day, luciferase was measured as a readout of infectivity. Created in https://BioRender.com . (B) Western blot of pelleted Spike (S), Nucleocapsid (N), Membrane (M), Envelope (E) in unexposed virus particles. The image is representative of two independent experiments. (C) SC2-VLP infectivity assay. Frequencies (1–6 GHz) were tested over a range of exposure times (2–10 min). Data represent compiled mean +/- SD of 5 independent experiments. The luciferase signal for each frequency/exposure time was performed in triplicate. P-values indicate Wilcoxon matched-pair signed rank test of unexposed compared to exposed samples. ( n = 90 (Unexposed); n = 36 (1.0–2.5 GHz & 2.5–3.5 GHz) n = 15 (3.5–4.8 GHz), n = 33 (4.8–6 GHz), n = 18 (MW)). RLU: Renilla Luciferase Units (RLU). MW: Microwave.
Protein Reconstitution Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein reconstitution buffer/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
protein reconstitution buffer - by Bioz Stars, 2026-03
99/100 stars
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Image Search Results


The SDS–PAGE results confirm similar levels of protein reconstitution in each liposome preparation. The gel is one representative result from at least two independent experiments.

Journal: Life Science Alliance

Article Title: Cryo-EM structures reveal the H + /citrate symport mechanism of Drosophila INDY

doi: 10.26508/lsa.202402992

Figure Lengend Snippet: The SDS–PAGE results confirm similar levels of protein reconstitution in each liposome preparation. The gel is one representative result from at least two independent experiments.

Article Snippet: The dried lipids were resuspended in reconstitution buffer containing 20 mM MES-NaOH pH 6.0, and 50 mM NaCl for [ 14 C]-citrate transport assay, or 20 mM MES-NaOH pH 6.0, 150 mM KCl, and 1 mM pyranine (trisodium 8-hydroxypyrene-1,3,6-trisulfonate; MedChemExpress) for H + transport assay.

Techniques: SDS Page

Electromagnetic waves reduce infectivity of SARS-CoV-2 Virus-Like Particles (SC2-VLPs). (A) Diagram of SC2-VLP production, electromagnetic wave treatment and infectivity assay. Plasmids expressing each of the SARS-CoV-2 structural proteins (Nucleocapsid (N); Matrix (M); Envelope (E); and Spike (S)), or a plasmid encoding a packaging signal and luciferase transcript (Luc-PS9) were transfected into viral producer cells (HEK293T). SC2-VLPs were then collected, left unexposed or exposed to various electromagnetic wave frequencies (1–6 GHz) and used to infect target cells (HEK293T-ACE2-TMPRSS2). The next day, luciferase was measured as a readout of infectivity. Created in https://BioRender.com . (B) Western blot of pelleted Spike (S), Nucleocapsid (N), Membrane (M), Envelope (E) in unexposed virus particles. The image is representative of two independent experiments. (C) SC2-VLP infectivity assay. Frequencies (1–6 GHz) were tested over a range of exposure times (2–10 min). Data represent compiled mean +/- SD of 5 independent experiments. The luciferase signal for each frequency/exposure time was performed in triplicate. P-values indicate Wilcoxon matched-pair signed rank test of unexposed compared to exposed samples. ( n = 90 (Unexposed); n = 36 (1.0–2.5 GHz & 2.5–3.5 GHz) n = 15 (3.5–4.8 GHz), n = 33 (4.8–6 GHz), n = 18 (MW)). RLU: Renilla Luciferase Units (RLU). MW: Microwave.

Journal: Scientific Reports

Article Title: Electromagnetic waves destabilize the SARS-CoV-2 Spike protein and reduce SARS-CoV-2 Virus-Like particle (SC2-VLP) infectivity

doi: 10.1038/s41598-025-01896-1

Figure Lengend Snippet: Electromagnetic waves reduce infectivity of SARS-CoV-2 Virus-Like Particles (SC2-VLPs). (A) Diagram of SC2-VLP production, electromagnetic wave treatment and infectivity assay. Plasmids expressing each of the SARS-CoV-2 structural proteins (Nucleocapsid (N); Matrix (M); Envelope (E); and Spike (S)), or a plasmid encoding a packaging signal and luciferase transcript (Luc-PS9) were transfected into viral producer cells (HEK293T). SC2-VLPs were then collected, left unexposed or exposed to various electromagnetic wave frequencies (1–6 GHz) and used to infect target cells (HEK293T-ACE2-TMPRSS2). The next day, luciferase was measured as a readout of infectivity. Created in https://BioRender.com . (B) Western blot of pelleted Spike (S), Nucleocapsid (N), Membrane (M), Envelope (E) in unexposed virus particles. The image is representative of two independent experiments. (C) SC2-VLP infectivity assay. Frequencies (1–6 GHz) were tested over a range of exposure times (2–10 min). Data represent compiled mean +/- SD of 5 independent experiments. The luciferase signal for each frequency/exposure time was performed in triplicate. P-values indicate Wilcoxon matched-pair signed rank test of unexposed compared to exposed samples. ( n = 90 (Unexposed); n = 36 (1.0–2.5 GHz & 2.5–3.5 GHz) n = 15 (3.5–4.8 GHz), n = 33 (4.8–6 GHz), n = 18 (MW)). RLU: Renilla Luciferase Units (RLU). MW: Microwave.

Article Snippet: The cells were then transferred to an opaque white 96-well flat-bottom plate, mixed with 50 μL reconstituted luciferase assay buffer (Promega), and luciferase measured immediately on a plate reader (Biotek Synergy H1).

Techniques: Infection, Virus, Expressing, Plasmid Preparation, Luciferase, Transfection, Western Blot, Membrane